翻訳と辞書 Words near each other ・ Helicodiceros ・ Helicodictyon ・ Helicodiscidae ・ Helicodiscus ・ Helicargo ・ Helicarion ・ Helicarion australis ・ Helicarion leopardina ・ Helicarion mastersi ・ Helicarion rubicundus ・ Helicarionidae ・ Helicarionoidea ・ Helicarrier ・ Helicascus ・ Helicase ・ Helicase-dependent amplification ・ Helicat ・ Helice (genus) ・ Helice tridens ・ Helicella ・ Helicella itala ・ Helicella orzai ・ Helicella stiparum ・ Helicella striatitala ・ Helicene ・ Helichochaetus ・ Helichrysopsis ・ Helichrysum ・ Helichrysum aciculare ・ Helichrysum arachnoides Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Helicase-dependent amplification ： ウィキペディア英語版 Helicase-dependent amplification Helicase-dependent amplification (HDA) is a method for ''in vitro'' DNA amplification like the polymerase chain reaction (PCR), but that works at constant temperature. ==Introduction== The polymerase chain reaction is the most widely used method for ''in vitro'' DNA amplification for purposes of molecular biology and biomedical research. This process involves the separation of the double-stranded DNA in high heat into single strands (the denaturation step, typically achieved at 95–97 °C), annealing of the primers to the single stranded DNA (the annealing step) and copying the single strands to create new double-stranded DNA (the extension step that requires the DNA polymerase)requires the reaction to be done in a thermal cycler. These bench-top machines are large, expensive and costly to run and maintain, limiting the potential applications of DNA amplification in situations outside the laboratory (e.g., in the identification of potentially hazardous micro-organisms at the scene of investigation, or at the point of care of a patient). ''In vivo'', DNA is replicated by DNA polymerases with various accessory proteins, including a DNA helicase that acts to separate the DNA by unwinding the DNA double helix. HDA was developed from this concept, using a helicase (an enzyme) to denature the DNA. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Helicase-dependent amplification」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.